Evolutionary relationship and structural characterization of the EPF/EPFL gene family.

Evolutionary relationship and structural characterization of the EPF/EPFL gene family.

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EPF1-EPF2 and EPFL9/Stomagen act antagonistically in regulating leaf stomatal density.The aim of this study was to elucidate the evolutionary functional divergence of EPF/EPFL family genes.Phylogenetic analyses showed that AtEPFL9/Stomagen-like genes are conserved only in vascular plants and are closely ps5 price new jersey related to AtEPF1/EPF2-like genes.

Modeling showed that EPF/EPFL peptides share a common 3D structure that is constituted of a scaffold and loop.Molecular dynamics simulation suggested that AtEPF1/EPF2-like peptides form an additional disulfide bond in their read more loop regions and show greater flexibility in these regions than AtEPFL9/Stomagen-like peptides.This study uncovered the evolutionary relationship and the conformational divergence of proteins encoded by the EPF/EPFL family genes.